By T.K. Ghose ,A. Fiechter, N.Blakebrough
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Additional info for Advances in Biochemical Engineering, Volume 009: Microbial Processes
2 X 107 a Alanine-grown cells. b Observed microscopically. + + - 0 34 Y. 2 Adhesive Force The adhesive force between cells and hydrocarbon was assessed by Miura et aI. , using Candida intermedia IFO 0761 and Candida tropicalis ATCC 20336 by centrifuging cells adsorbed onto hydrocarbon at different centrifugal forces for 3 minutes and thereafter counting the ceils that were desorbed from the hydrocarbon due to the centrifugation. The effect of a surface active agent on the adhesive force was investigated by adding 50 ppm of Tween 20 to the cells that were adsorbed onto hydrocarbon.
31 32 32 34 35 42 43 46 46 48 50 52 54 54 1. I n t r o d u c t i o n The mechanism of liquid hydrocarbon uptake by microorganisms represents an important problem. The kinetics of microbial growth on hydrocarbon are a basis for industrial development of hydrocarbon processes. The kinetic model for cell growth on hydrocarbon should be based on the mechanism of hydrocarbon uptake. e. the aqueous and the hydrocarbon phase. Numerous discussions have taken place concerning the mechanism of liquid hydrocarbon uptake by microorganisms.
Mechanism of Liquid Hydrocarbon Uptake by Microorganismsand Growth Kinetics 37 Utilization of the drop- and accommodation-form 14C-n-decanes was much higher than that of dissolved ~4C-n-decane, the latter being negligible as shown in Fig. 2 although sufficient dissolved 14C-n-decane remained in the medium after this measurement. COz, produced by C. decane, the latter again being negligible as shown in Fig. 3. ) :3,---60 Fig. 2. Comparison between the J4C-n-decanes uptake by C. ) 60 Fig. 3. Comparison between the t4C-CO2produced by C.
Advances in Biochemical Engineering, Volume 009: Microbial Processes by T.K. Ghose ,A. Fiechter, N.Blakebrough